With feedback-controlled output stability and up to a 100:1 linear dynamic range, the LDI is the ideal light source for a wide range of applications including spinning disk confocal microscopy, structured illumination microscopy, FRAP, photoactivation / photoconversion, and. With feedback-controlled output stability and up to a 100:1 linear dynamic range, the LDI is the ideal light source for a wide range of applications including spinning disk confocal microscopy, structured illumination microscopy, FRAP, photoactivation / photoconversion, and. In many research fields, the demand for miniaturized laser-induced fluorescence (LIF) detection systems has been increasing. This work has developed a compact LIF detector, employing a laser diode as the excitation source and a photodiode as the photodetector with an adjustable laser focal spot, to. Devices emitting light in the wavelength range of 635–1600 nm are commercially available, and blue diode lasers emitting radiation as short as 400 nm are in the process of being commercialized (2). The illumination spectrum is often narrow band with line widths as narrow as tens of megahertz and divergence as low as fractions of milliradians. Lasers are either utilized in free space, steered with. We used the Hamamatsu model PLP-01 picosecond light pulser as a 413-nm excitation light source for frequency-domain fluorescence measurements. In comparison with sync-pumped/cavity dumped/frequency-doubled dye lasers, the 413-nm PLP-01 shows a longer FWHM (40 ps), a similar pulse repetition rate. Each channel has a BNC input connector for up to 10 kHz TTL/analog modulation of the driving current and a BNC output connector for monitoring the driving current or for synchronization with other devices. Its laser safety features include a rear panel interlock connector, a master key switch and a.